Auto-oxidative changes in un-refrigerated goat meat
Abstract
Lipid peroxidation of biological samples may be assesed by different chemical and physical methods. However, those based on the measurement of malondialdehyde formed from the breakdown of endoperoxides during the last stages of the oxidation of a poly unsaturated fatty acid appear to be the most widely used. A simple and a highly sensitive spectrophotometric method for determining thiobarbituric acid reactive species (TBARS) as markers of lipid peroxidation was used. The method is based on the reaction between malondialdehyde and thiobarbituric acid to form a pink chromogen whose absorbance can be determined spectrophotometrically at 532nm.